Journal: Aging Cell

Article Title: HNRNPC Succinylation Influences the Neurodegeneration of Alzheimer's Disease Through YME1L1 ‐Mediated Mitochondrial Metabolism

doi: 10.1111/acel.70646

Figure Lengend Snippet: YME1L1 promotes neuronal mitochondrial damage after AD. (A) CCK8 assay for cell viability. (B) Flow cytometry for apoptosis detection. (C) TEM for mitochondrial assessment (The green arrow indicates normal mitochondria, and the red arrow indicates damaged mitochondria). (D) ATP level measurement. (E) JC‐1 staining for cellular mitochondrial membrane potential measurement. (F) MitoSOX fluorescence staining for cellular mitochondrial ROS analysis. n = 3. * p < 0.05; ** p < 0.01; *** p < 0.001; **** p < 0.0001.

Article Snippet: Intracellular superoxide levels were measured using the MitoSOX Red fluorescent probe (HY‐D1055; MCE, USA).

Techniques: CCK-8 Assay, Flow Cytometry, Staining, Membrane, Fluorescence

Journal: Aging Cell

Article Title: HNRNPC Succinylation Influences the Neurodegeneration of Alzheimer's Disease Through YME1L1 ‐Mediated Mitochondrial Metabolism

doi: 10.1111/acel.70646

Figure Lengend Snippet: YME1L1 induces neuronal mitochondrial damage and neuronal death following AD through OPA1 hydrolysis. (A) WB detection of OPA1 expression in hippocampal tissue ( n = 6). (B) WB detection of OPA1 expression. (C) CCK8 assay for cell viability. (D) Flow cytometry for apoptosis detection. (E) TEM for mitochondrial detection (The red arrow indicates damaged mitochondria). (F) ATP level detection. (G) JC‐1 staining for measuring mitochondrial membrane potential. (H) MitoSOX fluorescence staining for analyzing mitochondrial ROS. n = 3. * p < 0.05; ** p < 0.01; *** p < 0.001; **** p < 0.0001.

Article Snippet: Intracellular superoxide levels were measured using the MitoSOX Red fluorescent probe (HY‐D1055; MCE, USA).

Techniques: Expressing, CCK-8 Assay, Flow Cytometry, Staining, Membrane, Fluorescence

Journal: Aging Cell

Article Title: HNRNPC Succinylation Influences the Neurodegeneration of Alzheimer's Disease Through YME1L1 ‐Mediated Mitochondrial Metabolism

doi: 10.1111/acel.70646

Figure Lengend Snippet: HNRNPC succinylation modifies neuronal mitochondrial damage and neuronal death following AD via YME1L1. (A) PCR and WB detection of YME1L1, WB detection of OPA1. (B) CCK8 analysis of cell activity. (C) Flow cytometry to detect cell apoptosis. (D) TEM detection of mitochondria (The green arrow points to normal mitochondria, and the red arrow points to damaged mitochondria). (E) ATP level detection. (F) JC‐1 staining was used to measure the mitochondrial membrane potential of cells. (G) MitoSOX fluorescent staining analysis of mitochondrial ROS in cells. n = 3. * p < 0.05; ** p < 0.01; *** p < 0.001; **** p < 0.0001.

Article Snippet: Intracellular superoxide levels were measured using the MitoSOX Red fluorescent probe (HY‐D1055; MCE, USA).

Techniques: Activity Assay, Flow Cytometry, Staining, Membrane

Journal: Aging Cell

Article Title: HNRNPC Succinylation Influences the Neurodegeneration of Alzheimer's Disease Through YME1L1 ‐Mediated Mitochondrial Metabolism

doi: 10.1111/acel.70646

Figure Lengend Snippet: SIRT5 affects neuronal mitochondrial function by desuccinylating HNRNPC. (A) WB detection of YME1L1 and OPA1 expression. (B) CCK8 analysis of cell activity. (C) Flow cytometry detection of cell apoptosis. (D) TEM detection of mitochondrial morphology (The green arrow points to normal mitochondria, and the red arrow points to damaged mitochondria). (E) ATP level detection. (F) JC‐1 staining to measure cell mitochondrial membrane potential. (G) MitoSOX fluorescent staining analysis of mitochondrial ROS in cells. n = 3. * p < 0.05; ** p < 0.01; *** p < 0.001; **** p < 0.0001; ns stands for no significant difference.

Article Snippet: Intracellular superoxide levels were measured using the MitoSOX Red fluorescent probe (HY‐D1055; MCE, USA).

Techniques: Expressing, Activity Assay, Flow Cytometry, Staining, Membrane